Background:

• Research Associate, Royal Karolinska Institute, Sweden, 1968-69
• Research Associate, University of Houston, 1969-71
• Honorary Doctorates: Uppsala University (Sweden), 1991; Masaryk University (Czechoslovakia), 1992
• James B. Himes Merit Award of the Chicago Chromatography Discussion Group, 1983
• M.S. Tswett Medal and Award in Chromatography, 1984
• ACS Award in Chromatography, 1986
• Eastern Analytical Symposium Award in Chromatography, 1988
• ISCO Award in Biochemical Instrumentation, 1988
• Distinguished Faculty Research Lecture, Indiana University, 1988-89
• ACS Chemical Instrumentation Award by Division of Analytical Chemistry, 1988
• Fellow, American Association for the Advancement of Science, 1989
• Society of Analytical Chemists of Pittsburgh, Keene P. Dimick Award in Chromatography, 1990
• Third International Symposium on Supercritical Fluid Chromatography Award for Pioneering Work in the Development of SFC, 1990
• M. J. E. Golay Award and Medal in Chromatography, 1991
• ACS Award in Separations Science and Technology, 1992
• Anachem Award, 1992
• R&D 100 Award, 1993
• Jan E. Purkynje Memorial Medal (Czech Academy of Sciences), 1993
• R&D Magazine Scientist of the Year, 1994
• Russian Academy of Sciences, M. S. Tswett Memorial Medal, 1994
• Chromatographic Society of Great Britain, A. J. P. Martin Gold Medal, 1994
• Theophilus Redwood Lectureship (Analytical Division of the Royal Society of Chemistry), 1996
• Distinguished Teaching and Mentoring Award of the University Graduate School, IU, 1997
• 2000 Pittsburgh Analytical Chemistry Award, 1999
• Indiana University Distinguished Faculty Award, 1999
• Foreign Member, The Royal Society of Sciences (Sweden), 1999
• Colacro Merit Medal (Buenos Aires, Argentina), 2000
• Visiting Scientist, Department of Immunogenetics, Max Planck Institute, Tübingen, Germany, 1980
• Associate Member, Viking 1975 (Mars Lander) Science Team, 1972-75
• Chairman, 1980 Gordon Conference on Analytical Chemistry
• U.S. Coordinator, U.S.-Japan Joint Seminar on Microcolumn Separation Methods, 1982. Editorial Boards: Journal of Chromatography/Biomedical Applications, 1978-88
• Journal of High Resolution Chromatography, 1986-89
• Chromatographia, 1984-89
• Talanta, 1984-94; Analytical Letters, 1985-
• Journal of Microcolumn Separations, 1989-
• Chemtracts, 1989-91
• Chemical Research in Toxicology, 1989-92
• Research and Development Magazine, 1988-
• Electrophoresis, 1991-94
• Analytical Chemistry, 1994-97
• Eastern Analytical Symposium Award for Outstanding Achievements in the fields of Analytical Chemistry, 2001
• Dal Nogare Award for Excellence in Separation Science, 2004.

Our laboratory has long been involved with developing new high-resolution and ultra-sensitive bioanalytical separation techniques. Current research focuses on substantial improvement in resolution of complex biological mixtures and identification of the separated compounds through techniques such as electrospray mass spectrometry and matrix-assisted laser desorption/ionization mass spectrometry.

The most recent developmental aspects of our work include the preparation of highly efficient microcolumns for various forms of chromatography and capillary electrophoresis; and new detectors based on the laser technologies, imaging detectors, and other measurement principles. Techniques of ultrahigh sensitivity are being developed for new, challenging problems of biological chemistry.

In particular, capillary electrophoresis and electrochromatography combined with laser-induced fluorescence offer highly sensitive measurements at less than attomole (10-18 mole) levels. To facilitate ultrasensitive analyses of biological compounds by this technique, we have synthesized unique fluorogenic reagents that permit a spectral match with laser characteristics. Highly sensitive determinations of carbohydrates in complex mixtures are among the most pressing problems of glycobiology, a field now often referred to as the last great frontier of biochemistry. The analytical systems under development in this laboratory involve enzyme microreactors, unique fluorescent tags, and sophisticated instrumentation for proteomics and carbohydrate analysis, including various forms of mass spectrometry for sequencing, linkage analysis and a site of posttranslational modification.

Biochemically related investigations concern lipid peroxidation and its connection to the molecular mechanisms of aging and certain human diseases. For several years, our laboratory has also been active in the identification of mammalian pheromones and the biochemical aspects of olfaction. Olfactorily receptive proteins have now been isolated from mammalian tissues, and we are currently pursuing their structures, binding sites for pheromones, and the relevant molecular dynamic aspects. The membrane-bound receptor proteins are being probed for their interaction with pheromones and the consequent transmembrane signaling. Our research program gives graduate students opportunities to learn about modern analytical instrumentation and how to apply it to problems of biological significance.

Selected Publications:

Pheromones, Binding Proteins and Receptor Responses in Rodents. M.V. Novotny, Biochem.Soc. Transactions, 31, 117-122 (2003).

New Hyphenated Methodologies in High Sensitivity Glycoprotein Analysis. M. Novotny and Y. Mechref, J. Sep. Science, J. Sep. Sci., 28 1956-1968 (2005).

Combining lectin microcolumns with high-resolution separation techniques for enrichment of glycoproteins and glycopeptides. M. Madera, Y. Mechref and M. V. Novotny, Analytical Chemistry 77, 4081-4090 (2005).

Comprehensive Assessment of N-Glycans Derived from a Murine Monoclonal Antibody: a Case for Multimethodological Approach, Yehia Mechref, Jan Muzikar, and Milos V. Novotny, Electrophoresis 26, 2034-2046 (2005).

Stir Bar Sorptive Extraction: A New Quantitative and Comprehensive Sampling Technique for Determination of Chemical Signal Profiles from Biological Media, H.A. Soini, K.E. Bruce, D. Wiesler, F. David, P. Sandra, M.V. Novotny, J. Chem. Ecol. 31, 377-392 (2005)

A monolithic PNGase F enzyme microreactor enabling glycan mass mapping of glycoproteins by mass spectrometry, A. Palm and M.V. Novotny, Rapid Comm. Mass Spectrom. 19, 1730-1738 (2005).

Microdeposition Device Interfacing Capillary Electrochromatography and Microcolumn Liquid Chromatography with Matrix-assisted Laser Desorption/Ionization Mass Spectrometry. T. J. Tegeler, Y. Mechref, K. Boraas, J.P. Reilly and M.V. Novotny, Anal. Chem. 76, 6698-6706 (2004).

Temperature Dependent Backbone Dynamics of Major Urinary Protein-I Complexed with the Pheromone 2-sec-Butyl-4,5-dihydrothiazole, H. Krizova, L. Zidek, M.J. Stone, M.V. Novotny, V. Sklenar. Journal of Biomolecular NMR 28, 369-384 (2004).

Determination of Trace Isoflavonoid Phytoestrogens in Biological Materials by Capillary Electrochromatography. Jason A. Starkey, Yehia Mechref, C.K. Byun, Rosemary Steinmetz, Ora H. Pescovitz, Milos V. Novotny, Anal. Chem., 74 5998-6005 (2003).

Thermodynamic Analysis of Binding Between Mouse Major Urinary Protein-I and the Pheromone 2-sec-Butyl-4,5-dihydrothiazole. S.D. Sharrow, M.V. Novotny and M.J. Stone, Biochemistry 42. 6302-6309 (2003).

Structural Characterization of Oligosaccharides Using MALDI-TOF/TOF Tandem Mass Spectrometry. Yehia Mechref, Milos V. Novotny and Cheni Kirshnan, Anal. Chem.75, 4895-4903 (2003).

Microscale Nonreductive Release of O-Linked Glycans for Subsequent Analysis through MALDI/TOF Mass Spectrometry and Capillary Electrophoresis. Y. Huang, Y. Mechref and M.V. Novotny, Anal. Chem 73, 6063-6069, (2001).